solute carrier family 7 member 11  (Proteintech)

Journal: Biomedical Reports

Article Title: Peroxiredoxin 4 suppresses ferroptosis in esophageal squamous cell carcinoma by activating the phosphoinositide 3-kinase  signaling pathway

doi: 10.3892/br.2026.2133

Figure Lengend Snippet: PRDX4 is an important regulator of ferroptosis in ESCC cells. (A) Determination of MDA, LPO and GSH contents in KYSE270 cells after transfection with PRDX4 siRNA. (B) Western blot analysis of the protein levels of GPX4, SLC7A11 and PTGS2 in KYSE270 cells transfected with PRDX4 siRNA. (C) The relative protein levels of GPX4, SLC7A11 and PTGS2 in KYSE270 cells transfected with PRDX4 siRNA. (D) Determination of MDA, LPO and GSH contents in KYSE30 cells after transfection with pcDNA3.1-PRDX4. (E) Western blot analysis of the protein levels of GPX4, SLC7A11 and PTGS2 in KYSE30 cells transfected with pcDNA3.1-PRDX4. (F) The relative protein levels of GPX4, SLC7A11 and PTGS2 in KYSE30 cells transfected with pcDNA3.1-PRDX4. (G) Detection of the levels of MDA, LPO and GSH in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (H) Western blot analysis of the protein expression levels of GPX4, SLC7A11 and PTGS2 in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (I) The relative protein levels of GPX4, SLC7A11 and PTGS2 in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (J) Detection of the levels of MDA, LPO and GSH in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. (K) Western blot analysis of the protein expression levels of GPX4, SLC7A11 and PTGS2 in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. (L) The relative protein levels of GPX4, SLC7A11 and PTGS2 in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. ** P<0.01, *** P<0.001 and **** P<0.0001, indicate statistical significance. PRDX4, peroxiredoxin 4; ESCC, esophageal squamous cell carcinoma; siRNA, small interfering RNA; MDA, malondialdehyde; LPO, lipid peroxidation; GSH, glutathione; GPX4, glutathione peroxidase 4; SLC7A11, solute carrier family 7 member 11; PTGS2, prostaglandin-endoperoxide synthase 2; Fer-1, ferrostatin-1; ns, not significant.

Article Snippet: The primary antibodies were as follows: Anti-PRDX4 (1:2,000 dilution; cat. no. 10703-1-AP), anti-E-cadherin (1:20,000 dilution; cat. no. 20874-1-AP), anti-N-cadherin (1:2,000 dilution; cat. no. 22018-1-AP), anti-Vimentin (1:20,000 dilution; cat. no. 10366-1-AP), and anti-GPX4 (1:1,000 dilution; cat. no. 30388-1-AP; all from Proteintech Group, Inc.), anti-solute carrier family 7 member 11 (SLC7A11; 1:1,000 dilution; cat. no. DF12509; Affinity Biosciences, Ltd.), anti-prostaglandin-endoperoxide synthase 2 (PTGS2; 1:1,000 dilution; cat. no. 27308-1-AP; Proteintech Group, Inc.), anti-p-PI3K (1:1,000 dilution; cat. no. AP0427; ABclonal Biotech Co., Ltd.), anti-PI3K (1:1,000 dilution; cat. no. A19742; ABclonal Biotech Co., Ltd.), anti-p-AKT (1:2,000 dilution; cat. no. 28731-1-AP; Proteintech Group, Inc.), anti-AKT (1:2,000 dilution; cat. no. 10176-2-AP; Proteintech Group, Inc.) and anti-β-actin (1:2,000 dilution; cat. no. 20536-1-AP; Proteintech Group, Inc.).

Techniques: Transfection, Western Blot, Control, Expressing, Small Interfering RNA

Journal: Environmental Health Perspectives

Article Title: Effects of Extreme Heat Exposure on Heatstroke and Liver Injury in Mice: The Role of PPARα

doi: 10.1021/EHP.6c00267

Figure Lengend Snippet: Molecular insights into the regulation of ferroptosis by PPARα activation mice received PPARα agonist fenofibrate intervention before heatstroke induction, followed by a 9 h recovery period at 22 °C. A,B, Total iron (A) and Fe 2+ (B) levels in the liver ( n = 6). C,D, qPCR analysis of Hmox1 (C) and Fpn (D) in the liver ( n = 8). E–G, Representative bands (E) and analyzed protein levels of Hmox1 (F) and FPN (G) in the liver ( n = 4). H, qPCR analysis of Hamp in the liver ( n = 8). I–K, qPCR analysis of Tfr1 (I), Dmt1 (J) and Ncoa4 (K) in the liver ( n = 8). L, qPCR analysis of Gpx4 , Aifm2 and Nrf2 in the liver ( n = 8). M–Q, Representative bands (M) and analyzed protein levels of GPX4 (N), FSP1 (O), Nrf2 (P) and SLC7A11 (Q) in the liver ( n = 4). The numeric data are shown in Table S8 . Data are presented as means ± SEM and assessed through two-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001, compared with CC group; # p < 0.05, ## p < 0.01, ### p < 0.001, compared with HC group. Note: Hmox1, heme oxygenase 1; FPN, ferroportin; Tfr1, transferrin receptor 1; Dmt1, divalent metal transporter 1; Ncoa4, nuclear receptor coactivator 4; Aifm2, apoptosis-inducing factor, mitochondrial 2; GPX4, glutathione peroxidase 4; FSP1, ferroptosis suppressor protein 1; SLC7A11, solute carrier family 7, member 11; Nrf2, nuclear factor erythroid 2-related factor 2; SEM, standard error of the mean; ANOVA, analysis of variance.

Article Snippet: Equal amounts of liver protein (5–10 mg) were analyzed by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes, which were blocked with 5% skim milk for 1 h and incubated with following primary antibodies overnight at 4 °C:heme oxygenase 1 (Hmox1, #82206s, 1:1000), nuclear factor erythroid 2-related factor 2 (Nrf2, #12721, 1:1000), β-actin (#4970, 1:5000) from Cell Signaling Technology (Danvers, MA, USA); glutathione peroxidase 4 (GPX4, #ab125066, 1:1000) from abcam (Cambridge, UK); ferroportin (FPN, #NBP1-21502, 1:1000) from Novus Biologicals (Littleton, Colorado, USA); ferroptosis suppressor protein 1 (FSP1, #20886-1-AP, 1:1000) from Proteintech (Wuhan, China); solute carrier family 7, Member 11 (SLC7A11, #A2413, 1:1000) from ABclonal (Wuhan, China); and PPARα (#sc-398394, 1:1000) from Santa Cruz Biotechnology (Dallas, Texas, USA).

Techniques: Activation Assay

Journal: Environmental Health Perspectives

Article Title: Effects of Extreme Heat Exposure on Heatstroke and Liver Injury in Mice: The Role of PPARα

doi: 10.1021/EHP.6c00267

Figure Lengend Snippet: Hypothetical model for the role of PPARα in extreme heat-induced liver injury. The red arrow indicates hepatic changes in heatstroke mice. The blue arrow indicates effects of PPARα intervention in heatstroke mice. Note: PPARα, peroxisome proliferator-activated receptor α; FPN, ferroportin; GPX4, glutathione peroxidase 4; FSP1, ferroptosis suppressor protein 1; SLC7A11, solute carrier family 7, member 11; Nrf2, nuclear factor erythroid 2-related factor 2.

Article Snippet: Equal amounts of liver protein (5–10 mg) were analyzed by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes, which were blocked with 5% skim milk for 1 h and incubated with following primary antibodies overnight at 4 °C:heme oxygenase 1 (Hmox1, #82206s, 1:1000), nuclear factor erythroid 2-related factor 2 (Nrf2, #12721, 1:1000), β-actin (#4970, 1:5000) from Cell Signaling Technology (Danvers, MA, USA); glutathione peroxidase 4 (GPX4, #ab125066, 1:1000) from abcam (Cambridge, UK); ferroportin (FPN, #NBP1-21502, 1:1000) from Novus Biologicals (Littleton, Colorado, USA); ferroptosis suppressor protein 1 (FSP1, #20886-1-AP, 1:1000) from Proteintech (Wuhan, China); solute carrier family 7, Member 11 (SLC7A11, #A2413, 1:1000) from ABclonal (Wuhan, China); and PPARα (#sc-398394, 1:1000) from Santa Cruz Biotechnology (Dallas, Texas, USA).

Techniques: